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KMID : 0614620000360060736
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Korean Journal of Gastroenterology
2000 Volume.36 No. 6 p.736 ~ p.744
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Relationship between Global DNA Hypomethylation and Gastric Carcinogenesis
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Cho Hwoang-Lae
Chang Young-Woon
Rha Yeong-Ho
Kim Hyo-Jong
Lee Dong-Keun
Kim Kyung-Jin
Dong Seok-Ho
Kim Byung-Ho
Lee Joung-Il
Chang Rin
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Abstract
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Background/Aims: DNA hypomethylation has been observed in some human neoplasms and implicated as an important factor in carcinogenesis. This study was designed to determine whether DNA hypomethylation occurs during the process of gastric carcinogenesis and to evaluate the relationship between the degree of DNA hypomethylation and the stage of cancer.
Methods: A total of 49 subjects were classified histologically into normal controls (n=10), erythematous gastritis group (n=13), chronic atrophic gastritis group (n=13), and gastric cancer group (n=13). The extent of DNA methylation was assessed by incubating DNA from gastric mucosal tissue with (3H)-methyl-S-adenosylmethionine and Sss1 methylase. A higher incorporation between DNA and exogenous (3H)-methyl group reflected a lower degree of intrinsic methylation.
Results: Global DNA hypomethylation was increased according to the severity of disease as follows: normal (13,430 dpm/μg/DNA), erythematous gastritis (28,900 dpm/μg/DNA), chronic atrophic gastritis (67,850 dpm/μg/DNA), gastric cancer (83,490 dpm/μg/DNA) (p<0.0001). Global DNA methylation in chronic atrophic gastritis and gastric cancer was decreased significantly compared with that in erythematous gastritis, respectively (p<0.01, p<0.001). However, no difference in DNA methylation was observed between chronic atrophic gastritis and gastric cancer (p>0.05). DNA hypomethylation did not correlate with cancer stage. Helicobacter pylori infection did not affect the DNA methylation.
Conclusions: Global DNA hypomethylation occurs in the early stage of gastric carcinogenesis. The degree of DNA hypomethylation do not increase with progression of the stage of cancer.
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KEYWORD
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Gastric cancer, DNA hypomethylation
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